产品名称

Creatine Phosphokinase from bovine heart

别 称

ATP: Creatine N-Phosphotransferase, CPK, Creatine Kinase, Phosphocreatine phosphokinase

含 量

≥30 units/mg protein; Type III, salt-free

CAS  NO.

9001-15-4

MDL  number

MFCD00130876

适用范围

生物试剂

l  理化信息

外 观

冻干粉

l  生物学信息

生物活性/药理作用

Creatine kinase plays a key role in the energy bolism of cells with intermittently high and fluctuating energy requirements. Examples of such cells include cardiac or skeletal muscle cells and neural tissues of brain and retina. The enzyme catalyzes the reversible transfer of the phosphoryl group from phosphorylcreatine to ADP, in order to generate ATP.The molecular mass of the protein is found to be approximately 80,000 Da. It is made up of 2 subunits, each having a molecular weight of 40,000 ± 2000. The lighter subunit is present in larger amounts.

应 用

Creatine phosphokinase from bovine heart  has been used to investigate whether endothelial cell growth is stimulated by ischemic hearts.Creatine phosphokinase from bovine heart has also been used to evaluate the effect of high but nontoxic dietary intake of copper and selenium on bolism in calves. The product has been used for tATPase assay of myofibrillar protein isolated from rabbit. This assay evaluated the kinetic influence of bound creatine kinase (CK) on Ca₂₊ activated myosin ATPase. The product has also been used for the enzymatic hydrolysis of protein samples during tryptophan estimation by pyrolysis gas chromatography.

注 意

The use of 0.1% albumin in the reaction buffer is recommended to avoid inactivation due to dilution.

有效单位定义

One unit will transfer 1.0 μmole of phosphate from phosphocreatine to ADP per min at pH 7.4 at 30 °C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov