产品名称

DNA Polymerase I from Escherichia coli lysogenic for NM 964

一般描述

DNA polymerase I (holoenzyme) has 5′→3′ and  3′→5′ exonuclease activities in addition to its synthetic activity. This bifunctional activity enables the enzyme to use nicks or gaps in double stranded DNA as starting points for DNA synthesis. The 5′→3′ exonuclease activity degrades the DNA strand complementary to the template strand  beginning at the nick. DNA synthesis begins at the 3′-end of the nick and produces a new strand of DNA complementary to the template. The net result is the movement of the polymerase along the template strand (nick translation) until the DNA complementary to the template (from the site of the original nick to the 5′-end of the template strand) is replaced.

别 称

Kornberg Polymerase

含 量

5,000-15,000 units/mL

CAS  NO.

9012-90-2

分子量

mol wt109 kDa

MDL  number

MFCD00130924

适用范围

生物试剂

l  理化信息

外 观

甘油水的缓冲溶液

l  生物学信息

应 用

DNA Polymerase I from Escherichia coli has been used to study the effects of the anti-tumor drug cis-diaminedichloroplatinum (II) on the enzyme activity.

样品准备说明

To ensure recovery of product from its storage container, centrifuge prior to use.

有效单位定义

One unit converts 10 nanomoles of deoxyribonucleoside triphosphates into acid insoluble material in 30 min at 37°C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov