产品名称

Polynucleotide phosphorylase from Synechocystis sp.

一般描述

Polynuclotide phosphorlyase in spinach chloroplasts acts as a exonuclease and a poly(A) polymerase.

别 称

PNPase, Polyribonucleotide Nucleotidyltransferase

含 量

≥500 units/mg protein; recombinant, expressed in E. coli

MDL  number

MFCD00131992

适用范围

生物试剂

l  理化信息

外 观

无色溶液

l  生物学信息

生物活性/药理作用

Polynucleotide phosphorylase (PNPase) is a bifunctional enzyme with a phosphorolytic 3′ to 5′ exoribonuclease activity and a 3′-terminal oligonucleotide polymerase activity. Polynucleotide phosphorylase localizes to the intermembrane space of mitochondria and has a critical function in regulating mitochondrial homeostasis in human cells.

应 用

Polynucleotide phosphorylase has been used in a study to discover that a major function of PNPase is the synthesis of CDP.  It has also been used in a study to investigate the enzyme responsible for RNA 3′-tail synthesis in S. coelicolor.

有效单位定义

One unit will polymerize 1.0 μmole of ADP, releasing 1.0 μmole of inorganic phosphate in 15 minutes, at pH 9.1 at 37°C.

Supplied as a solution in 20 mM Hepes buffer pH 7.9, 0.1mM EDTA, 2mM DTT, 12.5 mM MgCl², 60mM KCl, 20% (w/v) Glycerol

l  存储

存储温度

−70°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov