产品名称

Esterase from porcine liver

别 称

Carboxyl esterase, Carboxylic-ester hydrolase, PLE

含 量

≥150 units/mg protein (biuret)

CAS  NO.

9016-18-6

MDL  number

MFCD00131030

适用范围

生物试剂

l  理化信息

外 观

白色至浅棕色硫酸铵悬浮液

物理形态

Suspension in 3.2 M (NH⁴)²SO⁴, pH 8

l  生物学信息

生物活性/药理作用

Esterase acts on water-soluble carboxyl esters containing short chain fatty acids. Its functionality is attributed to the catalytic triad of Ser, His and Asp/Glu. Pig liver esterase catalyzes enantioselective conversion of an ester to a carboxylic acid. The molecular weight is found to be168 kDa. It is a serine enzyme with two active sites on each molecule, which dissociates into active half-molecules in the presence of dilute acid or concentrated salts.

应 用

Porcine liver esterase is used to catalyze  the hydrolysis of pentaacetyl catechin and epicatechin for use in pharmaceutical and industrial applications. Pig liver esterase is commonly used for kinetic resolutions and assymetric synthesis in organic chemistry. Esterase from porcine liver has been used in a study to assess the reduction of complex coronary atherosclerotic plaque development. Esterase from porcine liver has also been used in a study to investigate the effect of diabetes on bone marrow and circulating porcine endothelial progenitor cells. The enzyme from Sigma has been used to develop an alternative gel-d protocol for screening for bead-bound catalytic activity in aqueous media. The product has also been used to investigate the properties of the compound L-valine-3-{8-[(E)-2-[3-methoxyphenyl)ethenyl]-7-methyl-1-propargylxanthine-3-yl}propyl ester hydrochloride (MSX-4, an aminoacid ester prodrug of the adenosine A2A receptor antagonist MSX-2).

有效单位定义

One unit will hydrolyze 1.0 μmole of ethyl butyrate to butyric acid and ethanol per min at pH 8.0 at 25°C.

l  存储

存储温度

2-8°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov