产品名称

Cholesterol Esterase from Pseudomonas sp.

别 称

Cholesterol Esterase from Pseudomonas fluorescens, Sterol-ester acylhydrolase

含 量

≥200,000 units/g protein

CAS  NO.

9026-00-0

分子量

mol wt ~300 kDa

MDL  number

MFCD00071052

适用范围

生物试剂

l  理化信息

外 观

冻干粉

l  生物学信息

生物活性/药理作用

Cholesterol esterase (CE) is a reversible enzyme that can hydrolyze or synthesize fatty acid esters of cholesterol and other sterols. Hydrolysis of water insoluble long chain fatty acid esters requires bile salt activation. Hydrolysis of water soluble esters of short chain fatty acids and lysophospholipids does not require activation by bile salts. It also hydrolyzes tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramide. The enzyme may have multiple functions in lipid and lipoprotein bolism, and atherosclerosis.

应 用

This enzyme is useful for enzymatic determination of total cholesterol when coupled with cholesterol oxidase in clinical analysis.

样品准备说明

Cholesterol esterase is soluble in 0.4M potassium ; phosphate, pH 7.0 (1mg/ml)

有效单位定义

One unit will hydrolyze 1.0μmole of cholesteryl oleate to cholesterol and oleic acid per min at pH7.0 at 37°C  in the presence of taurocholate.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov