产品名称

Cholesterol Esterase from bovine pancreas

别 称

Sterol-ester acylhydrolase

含 量

≥200 units/g protein

CAS  NO.

9026-00-0

MDL  number

MFCD00071052

适用范围

生物试剂

l  理化信息

外 观

白色至浅棕色冻干粉末

物理形态

This product is partially purified from bovine pancreas and is supplied as a white to light brown lyophilized powder containing ≥20% protein (biuret) and potassium phosphate.

l  生物学信息

生物活性/药理作用

Cholesterol esterase (CE) is a reversible enzyme that can hydrolyze or synthesize fatty acid esters of cholesterol and other sterols. Hydrolysis of water insoluble long chain fatty acid esters requires bile salt activation. Hydrolysis of water soluble esters of short chain fatty acids and lysophospholipids does not require activation by bile salts. It also hydrolyzes tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramide. This monomeric glycoprotein may have multiple functions in lipid and lipoprotein bolism, as well as in atherosclerosis. Its molecular mass is found to be 84 kDa.

应 用

Cholesterol esterase bound to membrane-associated heparin on brush border membranes aids in the transport of cholesterol and free fatty acid across the membrane. This enzyme is widely used in the determination of serum cholesterol in clinical laboratories. The enzyme from Sigma has been used to evaluate the inhibitory and antioxidant functions of the methanol extract of the Camellia sinensis leaves under in vitro conditions. The enzyme has also been used to digest human serum samples to confirm the presence and position of acyl esters of 7α-hydroxycholesterol. Enzyme responsible for the hydrolysis of many of the fatty acid esters of cholesterol.

Optimum pH range: 6-8

Activators: Bisphenol A diglycidyl ether, cAMP-dependent protein kinase type II, ethanol, methanol, n-butanol, phosphatidylcholine, phosphatidylethanolamine, sodium taurocholic acid

Inhibitors: Bisphenol A methacrylate, diisopropylfluorophosphate, enolase, Hg₂₊,sodium fluoride, phosphatidic acid, phosphatidylcholine, phosphatidylserine

样品准备说明

Cholesterol esterase is soluble in 0.4 M  potassium phosphate, pH 7.0 at 1 mg/mL concentration.

有效单位定义

One unit will hydrolyze 1.0 μmole of cholesteryl oleate to cholesterol and oleic acid per minute at pH 7.0 at 37°C in the presence of taurocholate.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov