产品名称

Cholesterol Esterase from Pseudomonas fluorescens

别 称

Sterol-ester acylhydrolase

含 量

≥10,000 units/g protein

CAS  NO.

9026-00-0

MDL  number

MFCD00071052

适用范围

生物试剂

l  理化信息

外 观

棕褐色冻干粉

溶解性(25℃)

0.4M potassium phosphate, pH7.0: soluble 1.0mg/mL

l  生物学信息

生物活性/药理作用

Cholesterol esterase (CE) is a reversible enzyme that can hydrolyze or synthesize fatty acid esters of cholesterol and other sterols. Hydrolysis of water insoluble long chain fatty acid esters requires bile salt activation. Hydrolysis of water soluble esters of short chain fatty acids and lysophospholipids does not require activation by bile salts. It also hydrolyzes tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramides. The enzyme may have multiple functions in lipid and lipoprotein bolism, as well as in atherosclerosis. Its molecular mass is approximately 129 kDa and the optimum pH range is 7.0-9.0. The enzyme is activated by cholic acid, glycocholic acid, BSA, Mg₂₊ and 0.3% (v/v) ™ X-100. Ag₊, Hg₂₊ and ionic detergents inhibit the activity of the enzyme.

应 用

Cholesterol esterase from Pseudomonas fluorescens has been used in an optimization study of components in enzymatic cholesterol reagents containing cholesterol oxidase. Cholesterol esterase from Pseudomonas fluorescens has also been used in a study to investigate the nondenaturing protein electrotransfer of the esterase activity of lipolytic preparations. This enzyme is widely used in the determination of serum cholesterol in diagnostic laboratories.

样品准备说明

Cholesterol esterase is soluble in 0.4M potassium phosphate, pH7.0 (1mg/mL).

有效单位定义

One unit will hydrolyze 1.0μmole of cholesteryl oleate to cholesterol and oleic acid per min at pH7.0 at 37°C  in the presence of taurocholate.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov