产品名称

Phospholipase A² from honey bee venom (Apis mellifera)

一般描述

Phospholipase A² from honey bee venom (Apis mellifera) is a calcium-dependent lipolytic enzyme.

别 称

Lecithinase A, PLA², Phosphatidylcholine 2-acylhydrolase

含 量

600-2400 units/mg protein; salt-free

CAS  NO.

9001-84-7

MDL  number

MFCD00131890

适用范围

生物试剂

l  理化信息

外 观

白色到灰色冻干粉

l  生物学信息

生物活性/药理作用

Hydrolyzes the β-ester bond of zwitterionic glycerophospholipids. Preferred substrates are phosphatidylcholine, phosphatidylethanolamine, and their plasmalogen analogues. Phosphatidylinositol and phosphatidylserine are also hydrolyzed. Aggressively attacks phospholipids in membranes of intact cells.

应 用

Phospholipase A² from honey bee venom (Apis mellifera) has been used

• to determine renal proximal tubular segments (PTS) viability during oxygenation and hypoxia-reoxygenation

• in the treatment of photosystem-II (PSII) dimer complex of Synechocystis to study changes in oxygen-evolving activity

• as a standard in phospholipase activity assay

样品准备说明

Sigma-Aldrich tests the solubility of this  product at 1.0mg/mL in water. One publication reports the preparation of stock solutions of this product at the equivalent of 17.5mg/mL in an aqueous buffer that contains 10mM MOPS, pH 7.2, 0.5mM EGTA,100mM HCl, and 4mM  CaCl²_.

有效单位定义

One unit will hydrolyze 1.0μmole of soybean L-α-phosphatidylcholine to L-α-lysophosphatidylcholine and a fatty acid per min at pH8.9 at 25°C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov