产品名称

Acetylcholinesterase from human erythrocytes

一般描述

Predominantly exists as a tetrameric glycoprotein composed of disulfide-ed homodimers with a monomer MW of ~80kDa.

别 称

AChE, Acetylcholine acetylhydrolase

含 量

≥500 units/mg protein (BCA)

CAS  NO.

9000-81-1

MDL  number

MFCD00081268

适用范围

生物试剂

l  理化信息

外 观

无色缓冲水溶液

物理形态

Solution in 20mM HEPES, pH8.0, containing  0.1% TRITON_® X-100

l  生物学信息

生物活性/药理作用

Acetylcholinesterase is the major in vivo degradative enzyme for acetylcholine. It converts acetylcholine and water to choline and acetic acid.Cholinesterases are inhibited by the natural carbamate alkaloid, eserine or physostigmine.

In blood there are two cholinesterases  present: The erythrocyte associated enzyme, which is a true cholinesterase or acetylcholinesterase [(AChE) - E.C. 3.1.1.7], the serum associated enzyme, which is Pseudocholinesterase or Butyrylcholinesterase [(BuChE) - EC  3.1.1.8].

AChE is an ectoenzyme, anchored to the erythrocyte membrane via a GPI moiety.

应 用

Acetylcholinesterase (AChE) from Sigma has been used in the structure-activity study of phosphoramido acid esters as inhibitors of AChE.

样品准备说明

The enzyme is the amphiphilic form extracted together with its GPI anchor with the aid of TRITON X-100 and purified by affinity chromatography.

有效单位定义

One unit will hydrolyze 1.0μmole of acetylthiocholine iodide per min at pH7.4 at 37°C.

l  存储

存储温度

2-8°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov