产品名称

Butyrylcholinesterase from equine serum

别 称

Acylcholine acyl-hydrolase, Choline esterase, butyryl, Pseudocholinesterase

含 量

≥500 units/mg protein

CAS  NO.

9001-08-5

MDL  number

MFCD00081455

适用范围

生物试剂

l  理化信息

外 观

冻干粉

物理形态

Highly purified, lyophilized powder containing buffer salts

l  生物学信息

生物活性/药理作用

Butyrylcholinesterase (BChE) is a serine hydrolase that is structurally similar to acetylcholinesterase (AChE), but differs in substrate specificities and inhibitor sensitivities. BChE can, unlike AChE, efficiently hydrolyze larger esters of choline such as butyrylcholine and benzoylcholine. The enzyme is a tetrameric glycoprotein with four equal subunits (110kDa each). The enzyme is activated by Ca₂₊ and Mg₂₊ and the activity is constant over the pH range 6.0-8.0. It is inhibited by Betaine, nicotine, organophosphates, carbamates.

应 用

Selective inhibition of BChE activity can be used in the detection of organophosphates. Its use in the treatment of organophosphate toxicity shows promise. There is a correlation between the level of BChE in human blood and degree of protection against potentially toxic nerve agents.There has also been interest in the roles of cholinesterases with regard to Alzheimer’s disease. Investigations into selective inhibitors may provide a clearer picture of the physiological role of BChE in both healthy and diseased individuals. The enzyme has been used to test bupivacaine as an inhibitor of butyrylcholinesterase during acetylcholinesterase assay using cerebrospinal fluid.

有效单位定义

One unit will hydrolyze 1.0μmole of  butyrylcholine to choline and butyrate per min at pH8.0 at 37°C. The  activity obtained using butyrylcholine as substrate is ~2.5 times that obtained using acetylcholine.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov