产品名称

Butyrylcholinesterase from equine serum

别 称

Acylcholine acyl-hydrolase,Choline  esterase, butyryl, Pseudocholinesterase

含 量

≥10 units/mg protein

CAS  NO.

9001-08-5

MDL  number

MFCD00081455

适用范围

生物试剂

l  理化信息

外 观

冻干粉

物理形态

Lyophilized powder containing buffer salts

溶解性(25°C)

cold water: 60 U/mL

l  生物学信息

生物活性/药理作用

Butyrylcholinesterase (BChE) is a serine hydrolase that is structurally similar to acetylcholinesterase (AChE), but differs in substrate specificities and inhibitor sensitivities. BChE can, unlike  AChE, efficiently hydrolyze larger esters of choline such as butyrylcholine  and benzoylcholine. The enzyme is a tetrameric glycoprotein with four equal subunits (110 kDa each). The enzyme is activated by Ca₂₊ and Mg₂₊ and the activity is constant over the pH range 6.0-8.0. It is inhibited by  betaine, nicotine, organophosphates, and carbamates.

应 用

Butyrylcholinesterase from equine serum has been used in a microcalorimetric study of the inhibition of butyrylcholinesterase by paraoxon. This enzyme has also been used in a study to investigate the synthesis and inhibition of cholinergic enzymes. Selective inhibition of BChE activity can be used in the detection of organophosphates. Its use in the treatment of organophosphate toxicity has shown clinical potential, as there is a correlation between the blood level of BChE in humans and the degree of protection against toxic nerve agents. There has also been an interest in the roles of cholinesterases with regard to Alzheimer’s disease. Investigations into selective inhibitors may provide a clearer picture of the physiological role of BChE in both healthy and diseased individuals. This product has been used for the screening of cholineesterase inhibitors in selected fruits and vegetables, for restoring cognitive function and improving memory. It has also been used to develop a butyrylcholinesterase and choline oxidase immobilized bio-sniffer for the detection of nicotine. Nicotine inhibits BChE activity. A decrease in the byproducts of BChE activity reflects the volume of nicotine.

样品准备说明

This product is prepared from equine serum using ammonium sulfate fractionation and supplied as a lyophilized powder.

有效单位定义

One unit will hydrolyze 1.0 μmole of  butyrylcholine to choline and butyrate per min at pH8.0 at 37°C. The activity obtained using butyrylcholine as substrate is ~2.5 times that obtained using acetylcholine.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov