产品名称

Phosphodiesterase II from bovine spleen

别 称

3′-Exonuclease, Spleen exonuclease, Spleen phosphodiesterase

含 量

≥5.0 units/mg protein

CAS  NO.

9068-54-6

MDL  number

MFCD00131884

适用范围

生物试剂

l  理化信息

外 观

冻干粉

l  生物学信息

生物活性/药理作用

Hydrolyzes RNA, RNA-Core, 3′-alkyl- and  3′-aryl-nucleoside phosphates, and polydeoxyribonucleotides with 3′-phosphate end groups to 3′-mononucleotides. Polynucleotides having 5′-phosphomonoester end groups are not attacked.

The enzyme acts on poly(A), poly(U), and poly(I). Native DNA and poly(C) are quite resistant to the action of this enzyme.

应 用

Phosphodiesterase (PDE) is any enzyme that is used to breaks phosphodiester bonds. It is a membrane-bound glycoprotein that is used to catalyze the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase II has been used in the enzymatic digestions of purified proteins such as the P8-dGMP complex. Bovine spleen phosphodiesterase has been used to digest N-cadherin. The product has been used in the characterization of polynucleotide chain length, composition, and identity of terminal nucleotide. The enzyme has also been used in excision of pyridyloxobutyl (POB) adducts from DNA. Furthermore, it has been used  along with micrococcal endonuclease to hydrolyze purified DNA to 3 -nucleoside monophosphates.

有效单位定义

One unit will produce acid soluble nucleotides equivalent to a ΔA²⁶⁰ of 16 in 30 min at pH6.5 at 37°C, in a  2.0mL reaction mixture.Substrate: RNA-Core. Actual A260 is measured on the supernatant after precipitation of the unhydrolyzed RNA with uranyl acetate-perchloric acid reagent.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov