产品名称

Deoxyribonuclease I bovine

别 称

DNAse I, Deoxyribonucleate 5′-oligonucleotido-hydrolase

含 量

≥5,000 units/mg protein; recombinant, expressed in Pichia pastoris

分子量

mol wt ~39 kDa

MDL  number

MFCD00130918

适用范围

生物试剂

l  理化信息

外 观

澄清缓冲的甘油水溶液

物理形态

Supplied as a solution in 4mg/ml glycine pH5.0, 5 mM calcium acetate and 50% glycerol

l  生物学信息

生物活性/药理作用

Digests single- and double-stranded DNA to a mixture of mono- and oligonucleotides carrying 5′ phosphates and 3′ OH termini. This catalytic activity is divalent ion-dependent. In the presence of Mg₂₊, DNase I hydrolyzes each strand of double-stranded DNA randomly and independently. In the presence of Mn₂₊,both strands can be cleaved.

DNase I is an endonuclease that acts on  phosphodiester bonds (adjacent to pyrimidines) to produce polynucleotides with terminal 5′-phosphates. The pH optimum is found to be between 7 and 8. Divalent cations such as Mn₂₊,Ca₂₊, Co₂₊, and Zn₂₊ are activators of the enzyme. A concentration of 5 mM Ca₂₊ stabilizes the enzyme against proteolytic digestion. 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS) and actin are known to inhibit the enzyme activity.

应 用

Used for the removal of DNA from protein samples.

DNAse I from Sigma was used to treat nuclear lysate to obtain single nucleosomes in a study. The enzyme has also been for the preparation and harvest of mice mammary glands.

Deoxyribonuclease I bovine has been used in a study to compare the initial actions of spleen deoxyribonuclease and pancreatic deoxyribonuclease. Deoxyribonuclease I bovine has also been used in a study to investigate deoxythymidine 3′, 5′-di-p-nitrophenyl phosphate as a synthetic substrate for bovine pancreatic deoxyribonuclease.

样品准备说明

Produced without using any animal cells or animal derived materials.

有效单位定义

One unit will produce a ΔA²⁶⁰ of 0.001 per min per mL reaction mixture using calf thymus DNA at pH5.0 and 25°C

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov