产品名称

Deoxyribonuclease I RNase-free solution  from bovine pancreas

一般描述

Digests single- and double-stranded DNA to a mixture of mono- and oligonucleotides carrying 5′ phosphates and 3′ OH termini. This catalytic activity is divalent ion-dependent. In the presence of Mg₂₊, DNase I hydrolyzes each strand of double-stranded DNA randomly and independently. In the presence of Mn₂₊,both strands can be cleaved.

含 量

5-15mg protein/ml; ≥10000un/mg protein

CAS  NO.

9003-98-9

分子量

mol wt 29.1 kDa

MDL  number

MFCD00130918

适用范围

生物试剂

l  理化信息

外 观

无色缓冲的甘油水溶液

l  生物学信息

应 用

Used in molecular biology applications for removing DNA during RNA purification, for preparing DNA for nick translation, and for DNA-protein interaction analysis by footprinting methods.

有效单位定义

One unit will cause a ΔA²⁶⁰ of 0.001 per min per mL reaction mixture using calf thymus DNA as substrate.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov