产品名称

Deoxyribonuclease II from porcine spleen

别 称

DNase II, Deoxyribonucleate 3′-oligonucleotido-hydrolase

含 量

2,000-6,000Kunitz units/mg protein (biuret); Type IV

CAS  NO.

9025-64-3

MDL  number

MFCD00130919

适用范围

生物试剂

l  理化信息

外 观

冻干粉

物理形态

Contains sodium chloride

l  生物学信息

生物活性/药理作用

The enzyme is a lysosomal acid hydrolase that hydrolyzes DNA to 3′-phosphoryl oligonucleotides in the absence of l ions, under acidic conditions. It is a non-covalently ed α·β heterodimer of approximately 45,000Da molecular mass.

应 用

DNase II from Sigma has been used to treat transformed cells during the purification of β-lactamase. It has also been used for the preparation of adenoma tissue in a study that investigated the effect of somatoprim on growth hormone secretion in human adenoma cell cultures (hSA).

Deoxyribonuclease II from porcine spleen has been used in an immunohistological study of the immune system cells in paraffin-embedded tissues. Deoxyribonuclease II from porcine spleen has also been used in a study to investigate its reassociation with the lysosomal membrane.

有效单位定义

One Kunitz unit will produce a ΔA²⁶⁰ of 0.001 per min per mL at pH 4.6 at 25°C; [Mg₂₊] = 0.83 mM

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov