产品名称

Deoxyribonuclease II from bovine spleen

别 称

DNase II, Deoxyribonucleate 3′-oligonucleotido-hydrolase

含 量

≥1,000 units/mg protein;Type V, essentially salt-free

CAS  NO.

9025-64-3

MDL  number

MFCD00130919

适用范围

生物试剂

l  理化信息

外 观

白色至浅棕色的冻干粉末

l  生物学信息

生物活性/药理作用

Deoxyribonuclease II, also called as acid DNAse, hydrolyzes deoxyribonucleotide ages in native and denatured DNA yielding products with 3′-phosphates. In vitro, its optimum pH range is 4.5 - 5.0. It also acts upon p-nitrophenyl-phosphodiesters at pH 5.6 - 5.9. The molecular weight is approximately 38,000 Da.

应 用

DNAse II from Sigma has been used in the dissociation medium during the preparation of embryonic cardiac myocytes from rat heart.Deoxyribonuclease II from bovine spleen has been used in a study that conducted a partial purification of deoxyribonucleases from eggs and liver of Xenopus laevis. Deoxyribonuclease II from bovine spleen has also been used in a study to investigate nucleic acid and protein synthesis of splenic lymphocytes.

有效单位定义

One Kunitz unit will produce a ΔA²⁶⁰ of 0.001 per min per mL at pH4.6 at 25°C; [Mg₂₊] = 0.83mM

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov