产品名称

Ribonuclease T1 from Aspergillus oryzae

别 称

Guanyloribonuclease, Ribonucleate 3′-guanylo-oligonucleotidohydrolase

含 量

300,000-600,000 units/mg protein

CAS  NO.

9026-12-4

MDL  number

MFCD00132191

适用范围

生物试剂

l  理化信息

外 观

无色至浅黄色至浅米色悬浮液

物理形态

Suspension in 2.8 M (NH⁴)²SO⁴ solution

l  生物学信息

生物活性/药理作用

Ribonuclease T1 (RNase T1) from Aspergillus oryzae is an endoribonuclease that hydrolyzes after G residues.Cleavage occurs between the 3’-phosphate group of a guanidine ribonucleotide and 5’-hydroxyl of the adjacent nucleotide. The initial product is a 2’:3’ cyclic phosphate nucleoside that is hydrolyzed to the corresponding 3’-nucleoside phosphate. It differs from Pancreatic RNase in that it attacks the guanine sites specifically to yield 3′-GMP and oligonucleotides with a 3′-GMP terminal group.

应 用

Ribonuclease T1 (RNase T1) from Aspergillus oryzae is used to digest denatured RNA prior to sequencing and is used for protein folding studies.

有效单位定义

One unit will produce acid soluble oligonucleotides equivalent to a ΔA²⁶⁰ of 1.0 in 15min at pH7.5  at 37°C, in a reaction volume of 1.0mL. Substrate: Yeast RNA.

l  存储

存储温度

2-8°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov