产品名称

Ribonuclease A from bovine pancreas

一般描述

RNase A is an endoribonuclease that attacks at the 3’OHphosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with single stranded RNA.

别 称

Pancreatic Ribonuclease, RNase A, Ribonucleate 3′-pyrimidinooligonucleotidohydrolase

含 量

Protein: 20-40 mg/mL; ≥Kunitz units/mg protein

CAS  NO.

9001-99-4

分子量

mol wt 13.7kDa; mol wt ~13,700

MDL  number

MFCD00132181

适用范围

生物试剂, for molecular biology

l  理化信息

外 观

无色至淡褐色液体

物理形态

Solution of 50% glycerol,10mM Tris-HCL pH8.0

l  生物学信息

应 用

Suitable for:

• RNase protection assays

• Removal of unspecifically bound RNA

• Analysis of RNA sequences

• Hydrolysis of RNA contained in protein samples

• Plasmid DNA purification

Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.

有效单位定义

A major application for RNase A is the removal of RNA from preparations of plasmid DNA. For this application,DNase free RNase A is used at a final concentration of 10 ug/mL. Boiling stock solutions of this RNase A product to inactivate residual DNase is not necessary and may cause precipitation of RNase and possible loss of enzymatic activity. If an RNase A solution is heated at a neutral pH, precipitation will occur. When heated at a lower pH, some precipitation may occur because of protein impurities that are present.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov