产品名称

Ribonuclease A from bovine pancreas

一般描述

Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can hydrolyze RNA from protein samples. Pancreatic RNase A specifically cleaves at the 3′-side of pyrimidine (uracil or cytosine)  phosphate bonds.

别 称

Pancreatic Ribonuclease, RNase A, Ribonucleate 3′-pyrimidinooligonucleotidohydrolase

含 量

≥90%(SDS-PAGE), ≥80Kunitz units/mg  protein; Type X-A

CAS  NO.

9001-99-4

分子量

mol wt ~13,700

MDL  number

MFCD00132181

适用范围

生物试剂

l  理化信息

外 观

无色至淡黄色透明至微浊液体

物理形态

Solution in 0.2 M sodium phosphate buffer, pH 6.4

l  生物学信息

应 用

Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays. Ribonuclease A is used to remove RNA from DNA  plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA. Ribonuclease A from bovine pancreas has been used in a study to assess the mechanism of heavy l ions on RNase activity. Ribonuclease A from bovine pancreas has also been used in a study to investigate the performance of oligomeric poly(diallyldimethylammonium chloride) as displacer for cation-exchange displacement chromatography of proteins.

样品准备说明

A further chromatographic purification of R5503 to yield essentially pure RNAse A

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov