产品名称

Phosphatase, Alkaline shrimp

一般描述

Shrimp alkaline phosphatase is a heat labile, hydrolase enzyme. It is a high specific activity alkaline phosphatase purified from a recombinant source. There are two preferred sites for enzyme activity: 5′ protruding, recessive and blunt 5′-termini. The enzyme is irreversibly heat inactivated at 65°C for 15minutes.

别 称

Orthophosphoric-monoester phosphohydrolase (alkaline optimum), Phosphatase, Alkaline from shrimp

含 量

≥1,000 DEA units/mL, recombinant, expressed in proprietary host

CAS  NO.

9001-78-9

MDL  number

MFCD00131849

适用范围

生物试剂

l  理化信息

外 观

甘油水的缓冲溶液

物理形态

solution in 50% glycerol containing 25mM Tris-HCl, pH7.5, 1mM MgCl²

l  生物学信息

生物活性/药理作用

Alkaline phosphatase is commonly responsible for dephosphorylation of nucleotides, proteins and alkaloids. The enzyme nonspecifically catalyzes the dephosphorylation of 5′-termini DNA and RNA phosphomonoesters reactions but it does not affect diphosphate or triphosphate ages.

应 用

Alkaline phosphatase was used to examine its role in the prevention of high-fat-diet-induced bolic syndrome in mice.It also may be used in pasteurization process for milks used in dairy products.

有效单位定义

One unit will hydrolyze 1μmole of 4-nitrophenyl phosphate per minute at pH9.8 at 37°C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov