产品名称

Phosphatase, Alkaline from bovine intestinal mucosa

别 称

Alkaline phosphatase, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

含 量

≥5,500 DEA units/mg protein

CAS  NO.

9001-78-9

分子量

dimer mol wt ~160 kDa

MDL  number

MFCD00131849

适用范围

生物试剂

l  理化信息

外 观

缓冲的甘油水溶液

物理形态

Solution in 50% glycerol containing 5mM Tris, 5mM MgCl² and 0.1mM ZnCl², pH7.0

l  生物学信息

生物活性/药理作用

The enzyme has a broad specificity for phosphate esters of alcohols, amines, pyrophosphate, and phenols. It is routinely used to dephosphorylate proteins and nucleic acids.

应 用

Alkaline phosphatase can be used to  dephosphorylate casein and other proteins. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase..

High specific activity grade recommended for antibody and protein conjugation.

样品准备说明

Dilute solutions of alkaline phosphatase should be prepared in 10mM Trizma-HCl, pH 8.0, 1-5 mM MgCl², and 0.1-0.2mM ZnCl². 50% Glycerol can be included for long-term storage at 2–8°C.

有效单位定义

One DEA unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH9.8 at 37°C. (One glycine unit is equivalent to ~3 DEA units)

l  存储

存储温度

2-8°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov