产品名称

Phosphatase, Alkaline from Escherichia coli

别 称

Orthophosphoric-monoester phosphohydrolase(alkaline optimum)

含 量

20-50 units/mg protein (in glycine buffer)

CAS  NO.

9001-78-9

MDL  number

MFCD00131849

适用范围

生物试剂

l  理化信息

外 观

甘油水的缓冲溶液

物理形态

Solution in 5mM Tris-HCl, pH7.4, 0.5mM  MgCl², 0.5mM ZnCl², 50%(w/v) glycerol

l  生物学信息

生物活性/药理作用

Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad specificity for phosphate esters.

应 用

Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. Product P4069 is provided as a buffered aqueous glycerol solution.

注 意

Alkaline phosphatase in 5mM EDTA, pH8, will be irreversibly heat inactivated at 75°C for 10 minutes.

有效单位定义

One unit will hydrolyze 1.0μmole of p-nitrophenyl phosphate per min at pH10.4 at 37°C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov