产品名称

Phosphatase, Alkaline from Escherichia coli

别 称

Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

含 量

30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)

CAS  NO.

9001-78-9

MDL  number

MFCD00131849

适用范围

生物试剂

l  理化信息

外 观

淡黄色至黄褐色悬浮液

物理形态

Suspension in 2.5M (NH⁴)²SO⁴

l  生物学信息

生物活性/药理作用

Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad specificity for phosphate esters. Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad specificity for phosphate esters.

应 用

Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. Product P4252 has been used for bulk digestion of tRNA .Product P4252 is provided in the ratio of 30-90 units/mg of protein.  Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. Product P4252 is an ammonium sulfate suspension in glycine buffer.

注 意

Presence of 0.1% SDS in the alkaline phosphatase assays will inhibit any nuclease contamination in the sample.

有效单位定义

One unit will hydrolyze 1.0μmole of p-nitrophenyl phosphate per min at pH10.4 at 37°C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov