产品名称

Phosphatase, Alkaline from calf intestine

一般描述

Alkaline phosphatase is a phosphomonoesterase that catalyzes the cleavage of terminal phosphates from nucleic acids. The free 5′-OH can be phosphorylated with polynucleotide kinase and γ32P-ATP to produce γ32P-end-labeled nucleic acids. Linearized cloning vectors can be prevented from recircularizing by dephosphorylation with alkaline phosphatase.

别 称

CIAP, CIP, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

含 量

≥10,000 units/mL

CAS  NO.

9001-78-9

分子量

mol wt ~80 kDa

MDL  number

MFCD00131849

适用范围

生物试剂

l  理化信息

外 观

透明无色甘油水的缓冲溶液

l  生物学信息

应 用

Commonly used to remove the 5′-terminal phosphate from nucleic acids during molecular cloning reactions.

有效单位定义

One unit will hydrolyze 1 μmole of p-nitrophenyl phosphate per min at 37°C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov