l 基本信息 |
产品名称 | Phosphatase, Alkaline from bovine intestinal mucosa |
一般描述 | The enzyme is a glycoprotein containing approximately 12% carbohydrate (6% hexoses and 6% other neutral sugars). Each molecule of alkaline phosphatase contains four zinc atoms and four disulfide bridges.Maximal activity with alkaline phosphatase is achieved in the
presence of magnesium. It catalyzes the hydrolysis of phosphate monoesters such as p-nitrophenyl phosphate, phenyl phosphate, phenolphthalein phosphate, α-glycerol phosphate, β-glycerol phosphate, 2-phosphorylglycerate, triosephosphate, glucose-6-phosphate, glucose 1-phosphate, fructose 1-phosphate, fructose 6-phosphate, adenosine 5-phosphate adenosine 3-phosphate, phosphoenolpyruvate, and β-nicotinamide adenine dinucleotide phosphate. Arsenate, cysteine, iodine, inorganic phosphate, pyrophosphate, diisopropyl phosphate, triphenylphosphate, diisopropyl fluorophosphate, and L-phenylalanine are some of the strong inhibitors of alkaline phosphatase. |
别 称 | Alkaline phosphatase, Orthophosphoric-monoester phosphohydrolase (alkaline optimum) |
含 量 | ≥2,000 DEA units/mg protein; ≥1.0
mg/mL | CAS
NO. | 9001-78-9 |
分子量 | dimer mol wt ~160 kDa | MDL
number | MFCD00131849 |
适用范围 | 生物试剂 |
l 理化信息 |
外 观 | 硫酸铵悬浮液 |
物理形态 | solution in 3.2M ammonium sulfate, 1mM MgCl2 and 0.1mM ZnCl2, pH7.0 |
l 生物学信息 |
生物活性/药理作用 | Alkaline phosphatase, from bovine intestinal mucosa, is most stable in the pH range 7.5-9.5. The enzyme has a broad specificity for phosphate esters of alcohols, amines, pyrophosphate, and phenols and it requires zinc, and magnesium or calcium divalent ions for activity. The enzyme has a broad specificity for phosphate esters of alcohols, amines, pyrophosphate, and phenols. It is routinely used to dephosphorylate proteins and nucleic acids. |
应 用 | Alkaline phosphatase can be used to dephosphorylate casein and other proteins. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation.DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase.. Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It is routinely used to dephosphorylate proteins, such as casein, and nucleic acids. It may be used for protein labeling when high sensitivity is required. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase. Product P5521 has been used to study GTP hydrolysis . High specific activity grade recommended for antibody and protein conjugation. The enzyme from Sigma has been used in the assay of phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate. It has also been used for alkaline phosphatase assays using a Nafion®-modified electrode. |
样品准备说明 | Affinity purified; This product is prepared from bovine intestinal mucosa. Dilutions should be made in 10mM Tris-HCl, pH8.0, 1-5mM magnesium chloride, 0.1-0.2mM zinc chloride, and 50% glycerol
and stored at 2-8°C. |
有效单位定义 | One DEA unit will hydrolyze 1μmole of 4-nitrophenyl phosphate per minute at pH9.8 at 37°C. (One glycine unit is equivalent to ~3 DEA units) |
l 存储 |
存储温度 | 2-8°C |
l 注意事项及免责声明 |
本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。 |
l 参考文献 |
1. http://www.drugbank.ca 2. https://ncit.nci.nih.gov 3. https://www.ncbi.nlm.nih.gov |
