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Phosphatase, Alkaline bovine

商品编号: S0363
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国外库存,货期2-4周,具体详情请联系销售人员。

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详细介绍

l  基本信息

产品名称

Phosphatase, Alkaline bovine

别 称

Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

含 量

≥15mg protein/ml (UV);

≥4000 units/mg protein;

recombinant, expressed in Pichia pastoris

CAS  NO.

9001-78-9

分子量

apparent mol wt 160 kDa

MDL  number

MFCD00131849

适用范围

生物试剂

l  理化信息

外 观

溶液

l  生物学信息

生物活性/药理作用

Alkaline phosphatase dephosphorylates proteins and nucleic acids.

Alkaline phosphatase is a dimer consisting of two equal subunits.The enzyme is a glycoprotein containing approximately 12% carbohydrate (6% hexoses and 6% other neutral sugars). Each molecule of  alkaline phosphatase contains four zinc atoms and four disulfide bridges.Maximal activity with alkaline phosphatase is achieved in the presence of magnesium. It catalyzes the hydrolysis of phosphate monoesters such as p-nitrophenyl phosphate, phenyl phosphate, phenolphthalein phosphate, α-glycerol phosphate, β-glycerol phosphate, 2-phosphorylglycerate, triosephosphate, glucose-6-phosphate, glucose 1-phosphate, fructose 1-phosphate, fructose 6-phosphate, adenosine 5-phosphate adenosine 3-phosphate, phosphoenolpyruvate, and β-nicotinamide adenine dinucleotide phosphate. Arsenate, cysteine, iodine, inorganic phosphate, pyrophosphate, diisopropyl phosphate, triphenylphosphate, diisopropyl fluorophosphate, and L-phenylalanine are some of the strong inhibitors of alkaline phosphatase. The enzyme is most stable in the pH range 7.5-9.5.2. The pH optimum for enzymatic activity is pH 8-10.

应 用

Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It is routinely used to dephosphorylate proteins and nucleic acids. It may be used for protein labeling when high sensitivity is  required. Alkaline phosphatase may be also be used to dephosphorylate the  5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be  tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after  dephosphorylation with alkaline phosphatase.Product P8361 has been used to dephosphorylate Dishevelled (Dvl) , which is a key component in the Wnt/β-catenin signaling pathway.

An assay, using this ALP enzyme, was  developed to assess the cryoprotective activity of two proteins CRP-1 and CRP-2. The enzyme has been used for inhibitory studies of cyclic nucleotide analogs that inhibit prostatic acid phosphatase using a fluorogenic assay. It  is commonly used as a "reporter" in detection systems, in which it  is conjugated to a protein (antibody, streptavidin, etc.) that specifically  recognizes a target molecule. Alkaline phosphatase has also been used to  dephosphorylate casein.

有效单位定义

One DEA unit will hydrolyze 1 μmole of  4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C.

l  存储

存储温度

2-8°C

l  注意事项及免责声明

本产品仅用于实验研究,不得作为药物使用,不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov