产品名称

Phosphatase, Acid from sweet potato

一般描述

Acid phosphatase from sweet potato is a phosphomonoesterase, which can appear in multiple molecular forms of similar  molecular mass but with different isoelectric points.

别 称

(Orthophosphoric-monoester phosphohydrolase [acid optimum] )

含 量

≥10.0 units/mg protein (modified Warburg-Christian); Secondary activity: ≤9.9 units/mg protein ATPase

CAS  NO.

9001-77-8

MDL  number

MFCD00131847

分子量

mol wt 110 kDa

适用范围

生物试剂

l  理化信息

外 观

硫酸铵悬浮液

 物理形态

Suspension in 1.8M (NH⁴)²SO⁴, 10mM MgCl², pH5.3

l  生物学信息

生物活性/药理作用

Acid phosphatases (APase) are a family of enzymes that non-specifically catalyze the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate at an optimum pH of 4 to 7 by the following reaction: APaseR-PO⁴ + H²O  --------------- R-OH + HOPO³ ₂₊Their function in the production, transport, and recycling of phosphate is critical for the bolic and energy  transduction processes of the cell. As a group, Apases may be as important as  kinases in regulatory processes.

应 用

Potato acid phosphatase is used as a dephosphorylating reagent in thiochrome assays for the detection of thiamin in biological samples. In this assay, it was shown to be more effective than acid phosphatases from wheat germ or α-amylase.

Sweet potato acid phosphatase has been used in a study as a pre-column enzyme reactor via a covalent bond with glutaraldehyde and aminopropyl controlled-pore glass. It has also been used in a study to investigate the use of tyrosine, histidine, and cysteine as ligands for Mn(III) in sweet potato phosphatase.

样品准备说明

APase is soluble in cold water (0.15–0.25unit/ml). Prepare solution immediately before use.

有效单位定义

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH4.8 at 37°C.

l  存储

存储温度

2-8°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov