产品名称

Phosphatase, Acid from potato

别 称

(Orthophosphoric-monoester phosphohydrolase acid optimum), Acid Phosphatase from potato

含 量

≥0.5 unit/mg solid

CAS  NO.

9001-77-8

MDL  number

MFCD00131847

适用范围

生物试剂

l  理化信息

外 观

冻干粉

l  生物学信息

生物活性/药理作用

Acid phosphatase is a cytoplasmic lysosomal hydrolase that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate.

应 用

Acid phosphatase is routinely used to dephosphorylate proteins and nucleic acids. Acid phosphatase is an acid hydrolase that normally resides in lysosomes. It is used as marker for the identification of lysosomes in subcellular fractionations. Acid phosphatase, has been used as a control in order to study how Mycobacterium bovis BCG effects the levels of acid phosphatase activity in alveolar macrophages.

样品准备说明

APase is soluble in cold water (0.15–0.25 unit/ml). Prepare solution immediately before use.

有效单位定义

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH4.8 at 37°C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.  https://www.ncbi.nlm.nih.gov