产品名称

5′-Nucleotidase human

一般描述

5′-Nucleotidase is an extracellular enzyme found in a wide variety of mammalian cells. It helps to accelerate the hydrolysis reaction of phosphate group from 5′ nucleotides, resulting in corresponding nucleosides.

别 称

CD73, NT5E, ecto-5′-nucleotidase

含 量

recombinant, expressed in CHO cells, vial  of 6-12 μg; ≥90% (SDS-PAGE)

CAS  NO.

9027-73-0

分子量

mol wt ~61 kDa by SDS-PAGE (reducing)

MDL  number

MFCD00130311

适用范围

生物试剂

l  理化信息

外 观

甘油溶液

物理形态

Supplied as a solution containing Tris, NaCl, CaCl², and 20% glycerol.

l  生物学信息

生物活性/药理作用

5′-nucleotidase, also known as CD73, is an extracellular enzyme that converts nucleoside-5′−monophosphates to nucleosides with a substrate preference of AMP. Native 5′-nucleotidase is a GPI-anchored protein whose exporession is upregulated by hypoxia. 5′-nucleotidase has many functions in vivo including the generation of extracellular adenosine.

5′-Nucleotidase has various clinical significances. It is a key molecule in the regulation of cancer cells proliferation, migration and invasion in vitro tumor angiogenesis, and tumor immune escape in vivo. Due to this important role, the enzyme is a potential target for cancer research. It is also involved in salvage of extracellular nucleotides and plays a key role in the control of tissue homeostasis.

有效单位定义

One unit will hydrolyze 1.0μmole of inorganic phosphorus from adenosine 5′-monophosphate per min at pH9.0 at 37°C.

l  存储

存储温度

−70°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov