产品名称

Nuclease S1 from Aspergillus oryzae

一般描述

The Nuclease S1 enzyme from Aspergillus oryzae has the ability to degrade single-stranded oligonucleotides composed of either deoxynucleotides or ribonucleotides.

别 称

Endonuclease S1

含 量

≥100,000 units/ml, for single-strand DNA/RNA digestion

CAS  NO.

37288-25-8

MDL  number

MFCD00131010

适用范围

生物试剂

l  理化信息

物理形态

Solution containing 30mM sodium acetate, 50mM NaCl, 1mM ZnCl², 50% glycerol, 2mg/ml protein

l  生物学信息

生物活性/药理作用

Nuclease S1 isolated from Aspergillus oryzae exhibits endo- and exolytic hydrolytic activity for the phosphodiester bonds of single-stranded DNA and RNA yielding 5’-phosphomononucleotide and 5’-phosphooligonucleotide end-products. It is used to digest non-annealed polynucleotide tails and hairpin loops in RNA and DNA duplexes and can be used to convert superhelical DNA to the linear form.

SI nuclease from Aspergillus oryzae can generate double-stranded DNA breaks in response to DNA nicks or abasic sites.

应 用

Nuclease S1 from Aspergillus oryzae has been used in a study to assess a biochemical method for mapping mutational alterations in DNA. It has also been used in a study to investigate the DNA damage and repair in a γ-irradiated rat brain tumor.

有效单位定义

One unit will cause 1.0 microgram of single-stranded nucleic acid to become perchloric acid soluble per minute at pH4.6 at 37°C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov