产品名称

Nuclease P1 from Penicillium citrinum

一般描述

Nuclease P1 from Penicillium citrinum is a zinc-dependent endonuclease that exhibits increased activity in the presence of low concentrations of urea.

别 称

Endonuclease P1

含 量

≥200 units/mg protein (E1%/280,  3′-5′-Phosphodiesterase); ≥1,000 units/mg protein 3′-nucleotidase    

CAS  NO.

54576-84-0

MDL  number

MFCD00131010

适用范围

生物试剂

l  理化信息

外 观

白色冻干粉

l  生物学信息

生物活性/药理作用

Catalyzes the nonspecific endonucleolytic cleavage of single stranded DNA and RNA to yield nucleoside 5′-phosphates and  5′-phosphooligonucleotides. It does not appreciably degrade double-stranded nucleic acids, especially in the presence of more than 400 mM sodium chloride at pH6.0.

应 用

Nuclease P1 from Penicillium citrinum has been used in a study to assess crystal structures using ammonium sulphate or polyethylene glycol 4000 as a precipitating agent. [3] It has also been used in a study to investigate a method for the direct sequence analysis 20-25 nucleotides from the terinini of 5′ or 3′ end group labeled RNA.

Nuclease P1 is used to improve the sensitivity of a 32P-labeling method for the detection of DNA adducts.

The enzyme has an optimal temperature of approximately 70°C, but for a long incubation, a temperature below 60°C is more suitable. It is stable in the pH range of 5 - 8.

有效单位定义

3′-5′-Phosphodiesterase: One unit will liberate 1.0μmole of acid soluble nucleotides from RNA per min at pH5.3 at  37°C. 3′-Nucleotidase: One unit will hydrolyze 1.0μmole of orthophosphate from 3′-AMP per min at pH7.2 at 37°C.

l  存储

存储温度

2-8°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov