产品名称

Nuclease(Endonuclease from Serratia marcescens)

含 量

≥250 units/μL, ≥90% (SDS-PAGE), recombinant, expressed in E.coli

CAS  NO.

9025-65-4

MDL  number

MFCD00131010

适用范围

生物试剂

l  理化信息

外 观

透明无色缓冲水性甘油溶液

物理形态

Solution in 50%glycerol containing 20mM Tris HCl, pH .0, 2mM MgCl², and 20mM NaCl.

l  生物学信息

生物活性/药理作用

Digests native or heat-denatured DNA and RNA.

Endonuclease from Serratia marcescens is a dimer of 30 kDa subunits with two essential disulfide bonds. This endonuclease attacks and degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) and is effective over a wide range of operating conditions. The optimum pH for enzyme activity is found to be  8.0-9.2. It completely digests nucleic acids to 5′- monophosphate terminated oligonucleotides 3 to 5 s in length. This is ideal for removal of nucleic acids from recombinant proteins and for applications where complete digestion of nucleic acids is desirable. It also reduces viscosity in protein extracts and prevents cell clumping. Pre-treatment of a protein sample improves its  resolution on 2D gel electrophoresis by eliminating any bound nucleic acids.

应 用

Used for the removal of nucleic acid from protein samples.

Endonuclease from Serratia marcescens, can be used to degrade all forms of DNA and RNA while having no proteolytic  activity. Benzonase nuclease can also be used to prepare proteins in microcalorimetric experiments.

The enzyme from Tomums has been used to limit cell clumping during the preparation of chimeric cell mixtures.It has also been used for the preparation of nuclear extracts by digesting DNA and releasing nuclear proteins intimately associated with DNA.

有效单位定义

One unit will digest sonicated salmon sperm DNA to acid-soluble oligonucleotides equivalent to a ΔA²⁶⁰ of 1.0 in 30 min at pH8.0 at 37°C (reaction volume 2.625ml).

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov