产品名称

Nuclease, ultrapure(Endonuclease from Serratia marcescens)

含 量

≥250 units/μL, ≥99%(SDS-PAGE), recombinant, expressed in E. coli, ultrapure grade

CAS  NO.

9025-65-4

MDL  number

MFCD00131010

适用范围

生物试剂

l  理化信息

外 观

透明无色缓冲水甘油溶液

物理形态

Solution in 50% glycerol containing 20 mM Tris HCl, pH8.0, 2mM MgCl², and 20mM NaCl.

l  生物学信息

生物活性/药理作用

Digests native or heat-denatured DNA and RNA.

Endonuclease from Serratia marcescens is a dimer of 30 kDa subunits with two essential disulfide bonds. This endonuclease attacks and degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) and is effective over a wide range of operating conditions. It completely digests nucleic acids to 5′-  monophosphate terminated oligonucleotides 3 to 5 s in length. This is ideal for the removal of nucleic acids from recombinant proteins. It can also be used for applications where complete digestion of nucleic acids is desirable. It also reduces viscosity in protein extracts and prevents cell clumping. Pre-treatment of a protein sample with this enzyme improves its resolution on 2D gel electrophoresis by eliminating any bound nucleic acids. The optimum pH for enzyme activity is found to be 8.0-9.2.

应 用

Used for the removal of nucleic acid from protein samples.

The enzyme has been used for an assay to detect the amount of Tf1 RNA that is protected from degradation by the nuclease, Benzonase. The Tf1 element of Schizosaccharomyces pombe is a long terminal repeat-containing retrotransposon that encodes functional protease, reverse tranase, and integrase proteins. It has also been used in the fractionation of nuclear pellet and nuclear extract obtained from cell  lysates.

Ultrapure benzonase nuclease, or Endonuclease from Serratia marcescens, has been used in a study to assess the effect of sodium dodecylbenzenesulfonate and antifoaming agents on the endonuclease activity of Serratia marcescens. Endonuclease from Serratia marcescens has also been used in a study to investigate the structure of chromatin subunits.

有效单位定义

One unit will digest sonicated salmon sperm DNA to acid-soluble oligonucleotides equivalent to a ΔA²⁶⁰ of 1.0 in 30 min at pH8.0 at 37°C (reaction volume 2.625 ml).

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov