产品名称

Nuclease micrococcal from Staphylococcus aureus

别 称

Endonuclease micrococcal, MNase, Micrococcal endonuclease, Micrococcal nuclease

含 量

100-300 units/mg protein; Protein, ≥40% E1%/280 (Balance primarily sodium citrate)

CAS  NO.

9013-53-0

MDL  number

MFCD00131731

适用范围

生物试剂

l  理化信息

外 观

白色至白色/黄色冻干粉末

溶解性(25°C)

Clear to Slightly Hazy;Colorless to Faint Yellow;0.90 - 1.10mg/ml

l  生物学信息

生物活性/药理作用

Cleavage of DNA by Nuclease micrococcal is greater at the 5′ sides of A or T nucleotides than at G or C.

Hydrolyzes 5′-phosphodiester bonds of DNA.

应 用

Nuclease from Staphylococcus aureus has  been used in a study to assess coagulase and heat-resistant strains found in animals. It has also been used in a study to investigate the characteristic of two genes in S. aureus that encode two thermostable  nucleases.

样品准备说明

Reconstitute to a concentration of 1unit per 5µl with water.

注 意

One unit will produce 1.0μmole of acid soluble polynucleotides from native DNA per min at pH8.8 at 37°C, d on EM/260 = 10,000 for the mixed nucleotides.

有效单位定义

One unit will produce 1.0μmole of acid soluble polynucleotides from native DNA per min at pH8.8 at 37°C, d on EM/260 = 10,000 for the mixed nucleotides.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov