产品名称

Phosphodiesterase I from Crotalus adamanteus venom

别 称

5′-Exonuclease, Oligonucleate 5′-nucleotidohydrolase

含 量

vial of ≥0.40 units, Purified

CAS  NO.

9025-82-5

MDL  number

MFCD00131883

适用范围

生物试剂

l  理化信息

外 观

白色至灰白色粉末

l  生物学信息

生物活性/药理作用

Phosphodiesterase I breaks phosphodiester bonds and catalyzes the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase I is released from eucaryotic plasma membranes by phosphatidylinositol-specific phospholipase C.

应 用

Phosphodiesterase (PDE) is any enzyme that is used to breaks phosphodiester bonds. It is a membrane-bound glycoprotein that is used to catalyze the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase I is used in phosphodiesterase activation assays to hydrolyze AMP. Product P3243 is from Crotalus adamanteus venom and is purified. Product P3243 has been used to hydrolyze tRNA with wyosine derivatives into mononucleosides.

样品准备说明

Purified via the method of Williams, et al. and further treated to inactivate contaminating 5′-nucleotidase activity.

有效单位定义

One unit will hydrolyze 1.0 μmole of bis(p-nitrophenyl) phosphate per min at pH8.8 at 37°C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov