产品名称

Phosphodiesterase 3′,5′-Cyclic Nucleotide Activator-deficient from bovine heart

别 称

3′,5′-Cyclic-nucleotide 5′-nucleotidohydrolase from bovine heart, PDE

含 量

Protein: ~75%; >0.1 units/mg protein (with added activator)

分子量

mol wt ~60 kDa

MDL  number

MFCD00131864

适用范围

生物试剂

l  理化信息

物理形态

冻干粉（含咪唑缓冲盐和硫酸镁）

l  生物学信息

生物活性/药理作用

Hydrolyzes the 3′,5′-phosphodiester bond in cyclic nucleotide monophosphates, such as cAMP and cGMP, to the corresponding nucleotide 5′-monophosphate.

The herbicide Atrazine was shown inhibit phosphodiesterase 3′,5′-Cyclic Nucleotide Activator-deficient from bovine heart.

应 用

Cyclic nucleotide phosphodiesterase has been investigated for its mechanism of activation as a function of calmodulin and Ca₂₊. It has also been used in a study to show that the binding of trifluoperazine to the activator of cyclic nucleotide phosphodiesterase increases the activity by over 10-fold.

For use in calmodulin assay. Activity is reduced to <50% without calmodulin present.

样品准备说明

Crude preparation which has been stripped of the protein activator, calmodulin.     

有效单位定义

One unit will hydrolyze 1.0μmole of  3′:5′,-cyclic-AMP to 5′-AMP per min at pH7.5 at 30°C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov