产品名称

Phosphodiesterase, 3′,5′-cyclic-nucleotide-specific from bovine brain

一般描述

Phosphodiesterase (PDE1) exists in two isoforms -PDE1A2 and PDE1B1. The enzyme has a role in the complex interaction  between cyclic nucleotides and Ca²⁺ second-messenger systems.

别 称

3′,5′-Cyclic-nucleotide 5′-nucleotidohydrolase from bovine brain, PDE

含 量

15-30 units/mg protein (in the presence of 0.03 mM Ca₂₊ and a saturating level (10 units per ml) of calmodulin); 1-5 units/mg protein (without added calmodulin)

CAS  NO.

9040-59-9

MDL  number

MFCD00131864

分子量

mol wt ~60 kDa

适用范围

生物试剂

l  理化信息

外 观

冻干粉

物理形态

Lyophilized powder containing Tris-HCl buffer salts and lactose

l  生物学信息

生物活性/药理作用

Hydrolyzes the 3′,5′-phosphodiester in cyclic nucleotide monophosphates, such as cAMP and cGMP, to the corresponding nucleotide 5′-monophosphate.

应 用

Phosphodiesterase, 3′,5′-cyclic-nucleotide-specific from bovine brain has been used to assay the  protein activator, calmodulin and in in vitro adenylate cyclase assay.

样品准备说明

Affinity purified preparation which has been depleted of calmodulin.

有效单位定义

One unit will hydrolyze 1.0 μmole of  3′,5′-cyclic-AMP to 5′-AMP per min at pH7.5 at 30°C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov