产品名称

Phospholipase C from Bacillus cereus

别 称

Lecithinase C, Lipophosphodiesterase I, PC-PLC, Phosphatidylcholine cholinephosphohydrolase

含 量

Protein: 5%-15%; ≥200 units/mg protein

CAS  NO.

9001-86-9

MDL  number

MFCD00131895

适用范围

生物试剂

l  理化信息

外 观

白色至浅黄色冻干粉末

物理形态

Lyophilized powder containing approx.10% protein. Remainder; trehalose, zinc sulfate, and potassium phosphate

溶解性(25°C)

H²O: soluble 1.0mg/mL, faintly hazy to hazy

l  生物学信息

生物活性/药理作用

Hydrolyzes the phosphate bond on phosphatidylcholine and other glycerophospholipids yielding diacylglycerol; this enzyme will also hydrolyze the phosphate bonds of sphingomyelin, cardiolipin, choline plasmalogen and ceramide phospholipids.

Phospholipase C is induced by thrombin and platelet-activating factor, forming1,2-diacylglycerol and phosphatidic acid. PLC hydrolyzes the phosphate bond on phosphatidylcholine and other  glycerophospholipids yielding diacylglycerol; this enzyme will also hydrolyze  the phosphate bonds of sphingomyelin, cardiolipin, choline plasmalogen and ceramide phospholipids.

应 用

Phospholipase C (PLC) is used to study adrenoceptor-mediated transmembrane signaling. It is used to degrade inositol-containing phospholipids. Phospholipase C from Bacillus has been used to study how flhF affects the export of PLC and other secretory virulence factors.

The enzyme has been used in the digestion of phosphatidylserine fraction of Schistosoma mansoni adult worms.

样品准备说明

Reconstitution with water (1mg/ml) will yield a slightly hazy to hazy solution.

有效单位定义

One unit will liberate 1.0μmole of water soluble organic phosphorus from L-α-phosphatidylcholine per min at pH7.3 at  37°C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov