产品名称

Phospholipase D from Streptomyces chromofuscus

一般描述

Phospholipase D (phosphatidylcholine cholinehydrolase, EC 3.1.4.4) is a ubiquitous enzyme with a molecular weight of ~ 50,000Da. It is expressed in a variety of plant tissues, few  microorganisms and some mammalian tissues.

别 称

Lecithinase D, Phosphatidylcholine phosphatidohydrolase

含 量

≥50,000 units/mL

CAS  NO.

9001-87-0

分子量

mol wt ~60 kDa

MDL  number

MFCD00131897

适用范围

生物试剂

l  理化信息

外 观

缓冲的甘油水溶液

物理形态

Solution in 100mM Tris/HCl, pH8.0, 10% glycerol (v/v), and 0.1% Triton X-100 (w/v).

l  生物学信息

生物活性/药理作用

Phospholipase D is active on sphingomyelins and lysophospholipids. Phospholipase D hydrolyzes the phosphate bonds of phospholipids  and sphingomyelin to give the corresponding phosphatidic acid.

Phospholipase D (PLD) catalyses the hydrolysis of phosphatidylcholines (PC) to yield phosphatidic acid (PA) and choline.PC-PLD activity is regulated by hormones, cytokines, neurotransmitters  and growth factors. Thus, it is considered to have a role in signal transduction in various cell types. Three vital lipid messengers produced by PLD plays a vital role in various crucial cellular processes and these lipids also have an essential role in downstream triglyceride and phospholipid synthetic pathway. PLD is essential for exocytosis in neurons and endocrine cells. In addition, it also plays a key role in phagocytosis.

应 用

Phospholipase D from Streptomyces chromofuscus has been used to cleave choline-containing phospholipids (PCs).

有效单位定义

One unit will release 1μmol of choline from L-α-phosphatidylcholine per hour at pH8.0 and 37°C.

l  存储

存储温度

−70°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov