产品名称

α-Amylase from porcine pancreas

一般描述

α-Amylase isolated from porcine pancreas is a glycoprotein. It is a single polypeptide chain of ~475 residues containing two SH groups and four disulfide bridges and a tightly bound Ca₂₊ necessary for stability. Chloride ions are necessary for activity and stability. The pH range for activity is 5.5 to 8.0, with the pH optimum at 7.

别 称

β-N-acetylglucosaminidase porcine placenta, PPA, al1,4 glucan-4-glucanohydrolase, alpha-Amylase, porcine pancreas α-amylase

含 量

Type VI-B, ≥5units/mg solid

MDL  number

MFCD00081319

适用范围

生物试剂

l  理化信息

外 观

白色至浅米色粉末

l  生物学信息

生物活性/药理作用

α-Amylase hydrolyzes the α-(1,4) glucan ages in polysaccharides of three or more α-(1,4) ed D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose. α -Amylase, from porcine pancreas, is a glycoprotein that consists of a single polypeptide chain of approximately 475 residues containing  2 SH groups and four disulfide bridges and a tightly bound Ca₂₊ necessary for stability. The pH optimum is at 7.

应 用

α-Amylase is used to hydrolyze α bonds of α-ed polysaccharides, such as starch and glycogen. α-Amylase, from Sigma, has been used in various plant studies, such as bolism studies in Arabidopsis .α-Amylase, from porcine pancreas, has been used to remove starch during the study of starch bolism and ripening in tomatoes .

样品准备说明

Sigma-Aldrich does not run a separate solubility test for this product. One publication reports preparation of solutions of  this product at 0.5mg/mL in phosphate- buffered saline (20 mM sodium phosphate, pH6.9, plus 6mM NaCl)

有效单位定义

One unit will liberate 1.0mg of maltose  from starch in 3 min at pH6.9 at 20°C.

l  存储

存储温度

2-8°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov