产品名称

α-Amylase from porcine pancreas

一般描述

Molecular mass: 51-54 kDa.

α-Amylase isolated from porcine pancreas is a glycoprotein. It is a single polypeptide chain of ~475 residues containing two SH groups and four disulfide bridges and a tightly bound Ca₂₊ necessary for stability. Chloride ions are necessary for activity and stability. The pH range for activity is 5.5 to 8.0, with the pH optimum at 7.

别 称

β-N-acetylglucosaminidase porcine placenta, PPA, al1,4 glucan-4-glucanohydrolase, porcine pancreas α-amylase

含 量

Type I-A, PMSF treated, saline suspension, 700-1400 units/mg protein (E1%/280)

MDL  number

MFCD00081319

适用范围

生物试剂

l  理化信息

外 观

白色至浅黄色盐水悬浮液

物理形态

Suspension in 2.9M NaCl solution containing 3mM CaCl².

l  生物学信息

生物活性/药理作用

α-Amylase hydrolyzes the α-(1,4) glucan ages in polysaccharides of three or more α-(1,4) ed D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose. α -Amylase, from porcine pancreas, is a glycoprotein that consists of a single polypeptide chain of approximately 475 residues containing 2 SH groups and four disulfide bridges and a tightly bound Ca₂₊ necessary for stability.

应 用

α-Amylase is used to hydrolyze α bonds of α-ed polysaccharides, such as starch and glycogen. α-Amylase has been used in various plant studies, such as bolism studies in Arabidopsis.

样品准备说明

2× crystallized

有效单位定义

One unit will liberate 1.0mg of maltose from starch in 3 min at pH6.9 at 20°C.

l  存储

存储温度

2-8°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov