产品名称

α-Amylase from Bacillus sp.

别 称

1,4-α-D-Glucan-glucanohydrolase

含 量

≥400units/mg protein (Lowry)

CAS  NO.

9000-90-2

MDL  number

MFCD00081319

适用范围

生物试剂

l  理化信息

外 观

粉末

溶解性(25℃)

H²O: soluble 1.0mg/mL

l  生物学信息

生物活性/药理作用

α-Amylase hydrolyzes the α-(1,4) glucan ages in polysaccharides of three or more α-(1,4) ed D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose. α -amylase, isolated from Bacillus subtilis is a dimer and has a monomer molecular weight of 48.9 kDa. α-Amylase is a lloprotein and contains atleast one mol of calcium ion. The presence of calcium ion increases the stability of the enzyme towards denaturation by heat, acid, or urea. The pH range for activity of this product is 5.0 to 7.5, with the optimum pH range being 6.0-7.0. This product is stable from pH5.0 to 10.0. The optimum temperature range is 65-75°C. The effective temperature range is up to 90°C.

应 用

α-Amylase is used to hydrolyze α bonds of α-ed polysaccharides, such as starch and glycogen. α-Amylase, from Sigma, has been used in various plant studies, such as bolism studies in Arabidopsis .α-has also been also used to study the ability of corn and starch to protect enzyme activity. The enzyme has been used to compare the effects of bacterial and fungal α-amylase and scalded flour on bread quality and shelflife. It has also been used to determine the optimal levels of these components in order to achieve maximum volume and bread freshness. Furthermore, it has been used to digest hydroxypropylated starch in Korean waxy rice cake to retard retrogradation.

样品准备说明

Tomums does not run a separate solubility test for this product. One publication reports preparation of stock solutions of this product at 5mg/mL in various buffers (glycine-HCl, pH3; sodium phosphate, pH7; and HEPES, pH8)

有效单位定义

One unit will liberate 1.0mg of maltose from starch in 3 min at pH6.9 at 20°C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov