产品名称

Chitosanase from Streptomyces griseus

别 称

Chitosan N-acetylglucosaminohydrolase

含 量

>50units/mg protein (Bradford); chitinase <1.0unit/mg protein

CAS  NO.

51570-20-8

MDL  number

MFCD00211797

适用范围

生物试剂

l  理化信息

外 观

白色冻干粉

物理形态

Lyophilized powder containing potassium phosphate buffer salts

溶解性(25°C)

H²O: soluble, clear to slightly hazy, colorless (10U/0.3mL)

l  生物学信息

生物活性/药理作用

Chitosanase catalyzes the endohydrolysis of β(1,4) ages between N-acetyl-D-glucosamine and D-glucosamine residues in partially deacetylated chitosan. Chitosanase from Streptomyces griseus is capable of hydrolyzing both chitosan and carboxymethyl cellulose. It is used for the lysis of cell walls of fungi belonging to the group Mucorales. It is found in several types of microorganisms.

应 用

Chitosanase from Streptomyces griseus has been used in a study to assess the effect of chitin sources on production of chitinase and chitosanase. Chitosanase from Streptomyces griseus has also been used in a study to investigate the effective production of chitinase and chitosanase by Streptomyces griseus HUT 6037 using colloidal chitin and various degrees of deacetylation of chitosan.

The enzyme from Sigma has been used for the hydrolysis of the crude cell wall preparation of oomycete Aphanomyces euteiches, a major parasite of legume plants. It has also been used for the enzymatic hydrolysis of the fully de-N-acetylated chitosan to get chitosan oligomer mixtures during the preparation of biocompatible chitosan-alginate gel.

样品准备说明

For long term solution storage, reconstitute vial with 50% glycerol solution.Dilute to working concentrations with a 0.1% BSA solution.

有效单位定义

One unit will release 1μmole of glucosamine from chitosan per min at pH5.0 at 37°C as measured in the fluorimetric assay of Osswald, et al. (1992).

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.  https://www.ncbi.nlm.nih.gov