产品名称

Chitinase from Streptomyces griseus

别 称

Chitodextrinase, Poly(1,4-β-[2-acetamido-2-deoxy-D-glucoside]) glycanohydrolase

含 量

≥200units/g solid

CAS  NO.

9001-06-3

MDL  number

MFCD00130771

适用范围

生物试剂

l  理化信息

物理形态

褐色到棕褐色冻干粉末(基本上是无盐的)

溶解性(25°C)

H²O: soluble 0.90-1.10mg/mL

l  生物学信息

生物活性/药理作用

Chitinase is an extracellular enzyme complex that degrades chitin and has a molecular mass of approximately 30 kDa.Chitin is degraded to N-acetyl-D-glucosamine in 2 enzymatic reactions. Firstly, chitobiose units are removed from chitin by chitodextrinase-chitinase. The second reaction involves N-acetyl-glucosaminidase-chitobiase, which cleaves the disaccharide to its monomer subunits (that comprise of N-acetyl-D-glucosamine). The optimum reaction temperature is 37°C.

应 用

Chitinase from Streptomyces griseus has been used to study the effect of the allosamidin on the regulatory system for chitinase production. It has also been used to study the enrichment of chitinolytic microorganisms. This enrichment was achieved by the isolation and characterization of a chitinase with antifungal activity against  phytopathogenic fungi. Chitinase from Streptomyces griseus has been used as a standard for chitinase assays using the enzyme derived from Absidia glauca and Petunia hybrida extracts.

有效单位定义

One unit will liberate 1.0mg of N-acetyl-D-glucosamine from chitin per hour at pH6.0 at 25°C in a 2 hour assay.

One new 1hour unit = approx. 50 old 48 hour units.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.  https://www.ncbi.nlm.nih.gov