产品名称

Chitinase from Trichoderma viride

别 称

N-acetyl-β-glucosaminidase and chitodextrinase

含 量

≥600units/g solid

MDL  number

MFCD00130771

适用范围

生物试剂

l  理化信息

外 观

棕黄色冻干粉

溶解性(25°C)

0.05M phosphate buffer, pH6.0: soluble 0.90-1.10mg/mL, faintly hazy to hazy (with particles)

l  生物学信息

生物活性/药理作用

Chitinase is a 30 kDa (approx.) extracellular enzyme complex that degrades chitin.Chitin is degraded to N-acetyl-D-glucosamine in 2 enzymatic reactions. Firstly, chitobiose units are removed from chitin by chitodextrinase-chitinase, a poly(1,4-β-[2-acetamido-2-deoxy-D-glucoside])-glycanohydrolase. The second reaction involves N-acetyl-glucosaminidase-chitobiase, which cleaves the disaccharide to its monomer subunits of N-acetyl-D-glucosamine. The enzyme may be classified into endo- and exochitinase. The endochitinase activity involves random cleavage at internal points in the chitin chain. The exochitinase activity consists of a progressive action which starts at the non-reducing end of chitin and releases chitobiose or N-acetyl-glucosamine units. The chitinolytic enzymes from T. viride are a mixture of extracellular chitinolytic enzymes, which exhibit exo- and endochitinase activities. The major activity was found to be that of N-acetyl-β-glucosaminidase.

应 用

Chitinase from Trichoderma viride has been used in a study to investigate the differential release of high mannose structural isoforms by fungal and bacterial endo-β-N-acetylglucosaminidases. Chitinase from Trichoderma viride has also been used in a study to investigate a hevein-like protein and a class I chitinase with antifungal activity from leaves of the paper mulberry.

Chitinase from Trichoderma viride has been used to digest chitin in purified sponge spicules during the study of sponge skeletons.

样品准备说明

The product is soluble in phosphate buffer,  pH6.1, (1mg/mL), yielding a clear to faint hazy, tan solution. One publication reports preparation of stock solutions of this product at 20mg/mL in 20mM sodium phosphate buffer, pH7.7 Another publication reports preparation of stock solutions of this product at 20mg/mL, in a buffer of 20mM sodium citrate with 1M sorbitol, pH5.8. 8 We have not tested either condition.

有效单位定义

One unit will liberate 1.0mg of N-acetyl-D-glucosamine from chitin per hour at pH6.0 at 25°C in a 2 hour assay.

One new 1hour unit = approx. 50 old 48 hour units.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov