产品名称

Lysozyme from chicken egg white

一般描述

Lysozyme from chicken egg white, is a protein that can show broad spectrum of antibacterial activity against gram positive and gram negative bacteria. Its antimicrobial activity can be enhanced by pretreatment of the target microbial cells with chelating agents like ethylenediaminetetraacetic acid (EDTA).

Lysozyme from chicken egg white, is a protein that can show broad spectrum of antibacterial activity against gram positive and gram negative bacteria. Its antimicrobial activity can be enhanced by pretreatment of the target microbial cells with chelating agents like ethylenediaminetetraacetic acid (EDTA).

别 称

Mucopeptide N-acetylmuramoylhydrolase, Muramidase

含 量

analytical standard, suitable for HPLC and GC

CAS  NO.

12650-88-3

分子量

single-chain mol wt 14.3 kDa Mr ~14000

MDL  number

MFCD00131557

适用范围

生物试剂

l  生物学信息

生物活性/药理作用

Lysozyme hydrolyzes β(1→4) ages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells  are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due  to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates  l ions in the outer bacterial membrane.

The enzyme is active over a broad pH range (6.0 to 9.0). At pH6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100M) than at pH9.2 (0.01 to 0.06M).

应 用

Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.

l  存储

存储温度

2-8°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov