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Lysozyme from chicken egg white

商品编号: S0464
图示 货号 库存 价格(¥) 数量

备注:

国外库存,货期2-4周,具体详情请联系销售人员。

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详细介绍

l  基本信息

产品名称

Lysozyme from chicken egg white

一般描述

Lysosyme is an enzyme found in egg white that breaks down the cell walls in bacteria enhancing the efficiency of DNA and protein extraction.

别 称

Mucopeptide N-acetylmuramoylhydrolase, Muramidase

含 量

10mg/mL; ≥400,000units/ml ;

(Ready to use solution:10mg/mL lysozyme in 25mM sodium acetate pH4.5 with 50% glycerol.)

CAS  NO.

12650-88-3

MDL  number

MFCD00131557

分子量

single-chain mol wt 14.3 kDa

适用范围

生物试剂

l  理化信息

外 观

无色液体

物理形态

Solution in 50% glycerol, 25mM Sodium  Acetate

l  生物学信息

生物活性/药理作用

Lysozyme hydrolyzes β(1→4) ages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin.Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan.Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates l ions in the outer bacterial membrane.

The enzyme is active over a broad pH range (6.0 to 9.0). At pH6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100M) than at pH9.2 (0.01 to 0.06M).

应 用

Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.

Suitable for the purification of both DNA and protein from bacteria.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究,不得作为药物使用,不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov