产品名称

Lysozyme from chicken egg white

别 称

Mucopeptide N-acetylmuramoylhydrolase, Muramidase

含 量

Purity: ≥98%(SDS-PAGE), Protein: ≥90%(composition,UV); ≥40,000units/mg protein

CAS  NO.

12650-88-3

MDL  number

MFCD00131557

分子量

single-chain mol wt 14.3 kDa

适用范围

生物试剂

l  理化信息

外 观

冻干粉

l  生物学信息

生物活性/药理作用

Lysozyme hydrolyzes β(1→4) ages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates l ions in the outer bacterial membrane.

The enzyme is active over a broad pH range (6.0 to 9.0). At pH6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100M) than at pH9.2 (0.01 to 0.06M).

应 用

Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.

样品准备说明

3× crystallized

有效单位定义

One unit will produce a ΔA⁴⁵⁰ of  0.001 per min at pH6.24 at 25°C, using a suspension of Micrococcus lysodeikticus as substrate, in a 2.6mL reaction mixture (1cm light path).

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.  https://www.ncbi.nlm.nih.gov