产品名称

Lysozyme from chicken egg white

别 称

Mucopeptide N-acetylmuramoylhydrolase, Muramidase

含 量

protein ≥90%, ≥40,000units/mg protein

CAS  NO.

12650-88-3

分子量

single-chain mol wt 14.3 kDa

MDL  number

MFCD00131557

适用范围

生物试剂

l  理化信息

外 观

白色至灰白色冻干粉末

溶解性(25°C)

10 mg/mL (H²O), Colorless, Clear to Slightly Hazy.

l  生物学信息

生物活性/药理作用

Lysozyme hydrolyzes β(1→4) ages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates l ions in the outer bacterial membrane.

The enzyme is active over a broad pH range (6.0 to 9.0). At pH6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100M) than at pH9.2 (0.01 to 0.06M).

应 用

Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.

Lysozyme from chicken egg white has been used for the extraction of genomic DNA from bacterial cells. It has been used as an external standard for MALDI-TOF (matrix assisted laser desorption ionization-time of flight) mass analysis.

样品准备说明

For E. coli cell lysis, use a freshly prepared lysozyme solution (10mg/ml) in 10mM Tris-HCl, pH8.0.1

有效单位定义

One unit will produce a ΔA⁴⁵⁰ of  0.001 per min at pH6.24 at 25°C, using a suspension of Micrococcus lysodeikticus as substrate, in a 2.6mL reaction mixture (1cm light path).

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov