产品名称

α(2→3,6,8,9) Neuraminidase from  Arthrobacter ureafaciens

别 称

Acyl-neuraminyl Hydrolase, Neuraminidase from Arthrobacter ureafaciens, Sialidase

含 量

Proteomics Grade, suitable for MALDI-TOF MS; ≥25U/vial

CAS  NO.

9001-67-6

MDL  number

MFCD00131711

适用范围

生物试剂

l  理化信息

外 观

冻干粉

l  生物学信息

应 用

Neuraminidase is an important deglycosylation enzyme capable of cleaving all non-reducing unbranched N-acetylneuraminic and N-glycolylneuraminic acid residues by hydrolysis of α(2→6), α(2→3), α(2→8), and α(2→9) ages (affinity in the order given). Branched sialic acids may also be cleaved with the use of high concentrations of enzyme and prolonged incubations. Desialylated glycoproteins may then be further characterized by treatment with various exoglycosidases resulting in partial or complete O-deglycosylation. SDS-PAGE and MALDI-TOF MS are typically utilized in purification, structural analysis, and sequencing process. These techniques also remove heterogeneity and charge from the glycoprotein.

有效单位定义

One unit will release 1nmole of 4-methylumbelliferone from 2-(4-methylumbelliferyl) α-D-N-acetylneuraminic acid per minute at pH5.5 at 37°C.

l  存储

存储温度

2-8°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov